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Museomics of *Carabus* giant ground beetles shows an Oligocene origin and *in situ* Alpine diversificationuse asterix (*) to get italics
Marie T. PAULI, Jeremy GAUTHIER, Marjorie LABEDAN, Mickael BLANC, Julia BILAT, Emmanuel F.A. TOUSSAINTPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"
2024
<p style="text-align: justify;">The development of museomics represents a major paradigm shift in the use of natural history collection specimens for systematics and evolutionary biology. New approaches in this field allow the sequencing of hundreds to thousands of loci from across the genome using historical DNA. HyRAD-X, a recently introduced capture method using bench-top designed probes, has proved very efficient for recovering genomic-scale datasets using natural history collection specimens. Using this technique at both the intra- and interspecific levels, we infer the most robust phylogeny to date for Arcifera, an ecologically and morphologically diverse clade of <em>Carabus</em> giant ground beetles. We successfully generated a genomic dataset of up to 1965 HyRAD-X loci for all described species, permitting inference of a robust dated phylogenomic tree for this clade. Our species delimitation and population genomic analyses suggest that the current classification for Arcifera is in line with its evolutionary history. Our results suggest an origin of Arcifera in the late Oligocene followed by speciation events during the warm mid-Miocene unlinked to Pleistocene glaciations. The dynamic paleogeographic history of the Palearctic region likely contributed to the diversification of this lineage with a relatively ancient colonization of the proto-Alps followed by <em>in situ</em> speciation where most species of Arcifera are currently found sometimes syntopically likely as a result of post-glaciations secondary contacts.</p>
https://github.com/JeremyLGauthier/Arcifera_phylogenyYou should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://
https://github.com/JeremyLGauthier/Arcifera_phylogenyYou should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://
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Arcifera; Beetle evolution; Carabinae; Historical DNA; HyRAD-X; Phylogenomics; Palearctic biogeography; Pleistocene glaciations
NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).
Insecta, Phylogeny, Systematics
⁠Joachim Schmidt [Schmidt@agonum.de], Carmelo Andujar [Candujar@ipna.csic.es], Arnaud Faille [Arnaud.faille@smns-bw.de], Tonatiuh Ramírez-Reyes suggested: Lines 105-108 explicitly mention which are the two species, Tonatiuh Ramírez-Reyes suggested: Lines 108-109 structure references and parentheses appropriately, Tonatiuh Ramírez-Reyes suggested: Line 324 indicate the beta value, Tonatiuh Ramírez-Reyes suggested: Figure 1 homogenize colors in pinned (< 30-year-old) No need for them to be recommenders of PCI Zool. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct
e.g. John Doe [john@doe.com]
2024-03-27 15:30:31
Felix Sperling